What kind of boundaries of DNA replication?

[biokit]

Now, a new study Florida State University for the first time to determine the boundaries of these units, the article published in the November 19 issue of Nature. This achievement will help people further understand the replication mechanism and related diseases, such as cancer.

Professor David Gilbert and postdoctoral Ben Pope who led the research team, in-depth analysis of the genetic material in the nucleus replication. Their work is part of a larger project ENCODE, the project bursts of four articles in this issue of the journal Nature, a comprehensive comparison of the similarities and differences between mouse and human genomes.

Before high-resolution study of chromosome conformation capture (Hi-C) found that early and late replication time corresponds to the three-dimensional chromatin compartments (chromatin compartment) on and off. Chromatin and this compartment there are still sub-structures, i.e., the topological domains associated TAD. This structure is quite conserved in different cell types, and its size is quite replication domain. Learn More About Mouse Cystatin C ELISA Kit http://www.cusabio.com/ELISA_Kit-73147/ service from CUSABIO.

This study identified a domain boundary replicated, clear their position in the 18 kinds of human cells, and 13 kinds of mouse cells. Overall, the replication domain boundaries and TAD boundary is almost one to one relationship. The researchers pointed out, TAD domain is a stable replication time-control unit.

"Identification of regulatory elements are the basis for understanding natural phenomena, and now we finally get a copy of the regulation unit of time, which is an important step forward," Gilbert said. Further research in this area, will bring valuable new clues for cancer therapy and stem cell therapy.

"In many species, the replication time of the program are quite conservative, which indicates that it has a crucial importance," Pope said. "And of cancer and other diseases, can interfere with the normal operation of this program."

Cell magazine recently published an important outcome about the replication of time. The researchers first identified in the regulation of DNA replication timing of quantitative trait loci, and pointed out that DNA replication scheme between people is not the same.